Role of E4Orf3 Protein in adenovirus Life cycle in correlatin with E1B55k and Promyelocytic Leukemia protein Isoform II

Abstract

Promyelocytic leukemia protein isoform II (PML-II) mRNA expression was interfered with anti-PML-II- specific mRNA to downregulate its expression to analyse the infection outcome in presence or absence of PML-II. PML-II transient knockdown gave an effective down regulation at the protein level then the transfected cells were challenged with adenovirus wild type (wt300) or Orf3- deleted mutant (InOrf3) to analyze the outcome of both viruses infection. DNA binding protein (DBP) expression increased in both types of infection with the removal of PML-II with more or less level of infection in both types of infection. Adenovirus yield was higher with the removal of PML-II in both types of wt or InOrf3 infection. Interferon treatment reduced the expression of early protein ( E1b55k) in InOrf3 infection but not in wt infection when PML-II was absent. To conclude, E4Orf3 removal reduces the virus yield at later time points of infection through effect on E1b55k in an interferon induced environment.